Salk Institute for Biological Studies *
SalkCoresCCMI Center for Cytometry and Molecular Imaging
 
 
The Becton-Dickinson FACS Vantage SE DiVa is a high-speed multi-color multi-laser cell sorter. Two scatter parameters and up to 8 fluorescence colors may be collected per cell. Excitation sources include 2 Coherent I-90 high-power argon-ion lasers which provide primary lines at ~350 nm, 458 nm, 476 nm, 496 nm, 488 nm and 514 nm. There is also a 35 mW helium-neon laser which provides 633 nm excitation and a 10 mW DPSS unit providing 561 nm. Becton-Dickinson DiVa™ digital electronics enable generation of pulse height, area and width for all 10 detected parameters. The instrument is equipped with B-D TurboSort™ allowing cell sorting at high speeds, up to 30,000 cells/sec. Regular or large cells may be sorted. DiVa™ electronics allows sorting of up to 4 populations simultaneously, or cells may be sorted into multiwell plates.