Salk CCMI: Flow Cytometry: LSR I troubleshooting

Neither cytometer nor computer will not turn on.: Make sure that UPS under bench is turned on.
Computer will not connect to cytometer: Restart computer
Cytometer status NOT READY: Allow to warm up for at least 5 minutes.
Cytometer status remains at STANDBY: Fluidics control not set to "RUN"; Tube is cracked; Check pressure in sheath tank (Open black tap, depress quick-connect center); Check level in sheath tank (pick up tank, compare weight with waste); Ensure that sheath tank lid is fitted properly; Stuck fluidics - turn to STANDBY and back to RUN
Status READY, but sample will not run: No cells in sample; Clumpy sample - filter it; Blocked SIP or flow cell - PURGE 3 times; Check pressure in sheath tank (Open black tap, depress quick-connect center); Check level in sheath tank (pick up tank, compare weight with waste); Ensure that sheath tank lid is fitted properly; Waste not venting - check waste line; Air bubbles in sheath filter - purge filter
Sample runs for a few seconds then stops: Clumpy sample - filter it; Blocked SIP or flow cell - PURGE x3; Waste not venting - check waste line; Air bubbles in sheath filter - purge filter
Very slow sample rate: Sample very dilute; Fluidics set to LOW; Fluidics fine control turned down (Note: 10-turn control)
No signal in FL4/FL5: UV laser not turned on
Weak signal in FL4/FL5: Cells not stained; Incorrect filter inserted; PMT voltage too low
No signal in APC/Cy5 channel: Pulse processing not turned on; P8 parameter not set to SSC-W
Indo-1 signal absent/poor: UV laser not turned on; Pulse processing not turned on; Pulse processing card 2 not set to FL4+FL5; Check filter in FL4 (should be 405 BP); Check filter in FL5 (should be 510 BP)