Salk CCMI: Flow Cytometry: LSR II troubleshooting

Neither cytometer nor computer will not turn on.: Make sure that UPS under bench is turned on.
Can't log in to computer.: Make sure you are logging on to the CCMI domain.; Make sure you put in the correct user name and password.
Computer will not connect to cytometer.: Restart Diva software.
Computer STILL will not connect to cytometer.: Restart Diva hardware: Open the "Cytometer Status" application (the red telephone on the Windows desktop) and press the Enter key a few times. If you get a prompt such as "-->", type "reboot" and press Enter. If this fails, cycle the power to the cytometer.
No PE signal.: Ensure the 561 nm laser is turned on.
No events.: Laser(s) not turned on.; Fluidics control not set to "RUN".; Tube is cracked.; Check level in sheath tank.; Ensure that sheath tank lid is fitted properly.; Stuck fluidics - PRIME.; No cells in sample.; Clumpy sample - filter it.; Blocked SIP or flow cell - Remove sample, and press PRIME.; Waste not venting - check waste line for flow.; Air bubbles in sheath filter - purge filter.
Sample runs for a few seconds then stops: Clumpy sample - filter it.; Blocked SIP or flow cell - PRIME.; Waste not venting - check waste line.; Air bubbles in sheath filter - purge filter.
Very slow sample rate.: Sample very dilute.; Fluidics set to LOW.; Fluidics fine control turned down.
Sample still will not run: Air bubbles in sheath filter - purge filter.; SIP blocked - Set fluidics to "HIGH". Run 100% bleach for 30 sec. followed by at least 2 changes of DI water for 1 minute.