Five lasers are provided, as detailed below. Lasers may be turned on as required.

Laser	Power	Detector optic	Number of detectors
488 nm	50 mW	Octagon	4
561 nm	100 mW	Octagon	5
640 nm	40 mW	Trigon	3
405 nm	100 mW	Trigon	3
355 nm	20 mW	Trigon	2

Fluorescence is delivered from the sample to the detectors via 5 fiber optics corresponding to the excitation laser. The fibers terminate in fixed-alignment assemblies known as trigons and octagons. Long-pass dichroics pass the signal around the assembly, extracting longer wavelengths first, and proceeding to shorter wavelengths. Detectors within a trigon or an octagon are labeled alphabetically, with detector "A" detecting the longest wavelength and detector "C" (trigon) or "H" (octagon) detecting the shortest.