|
|
Standard optical layout
| Laser |
Detector |
Dichroic |
Bandpass |
Effective
Range |
Fluors |
| 488 |
A |
670LP |
692/40 |
672-712 |
PerCP, PerCP-Cy5.5 |
| |
B |
505LP |
525/50 |
500-550 |
FITC, GFP, YFP |
| |
C |
- |
488/10 |
483-493 |
SSC |
| 561 |
A |
750LP |
780/60 |
750-810 |
PE-Cy7 |
| |
B |
685LP |
710/50 |
685-735 |
PE-Cy5.5 |
| |
C |
635LP |
660/20 |
650-670 |
PE-Cy5, PI, 7-AAD, mKate, mPlum |
| |
D |
600LP |
615/45 |
600-635 |
PE-TR, PI, TR, Alexa-594, mCherry, mKate |
| |
E |
- |
582/15 |
574-590 |
PE, Cy3, Alexa-{546,555,568}, Ds-Red, tdTomato |
| 640 |
A |
750LP |
780/60 |
750-810 |
APC-Cy7 |
| |
B |
685LP |
730/45 |
707-750 |
Alexa-{680,700} |
| |
C |
- |
670/30 |
655-685 |
APC, Cy5, Alexa-{633,647} |
| 405 |
A |
535LP |
560/40 |
535-580 |
Pacific Orange, Cascade Yellow |
| |
B |
505LP |
515/20 |
505-525 |
mCFP, Alexa-430 |
| |
C |
- |
450/50 |
425-475 |
Pacific Blue, Alexa-405, Cascade Blue |
| 355 |
A |
505LP |
525/50 |
500-550 |
Indo-1 (green[lo-ca]) |
| |
B |
- |
438/24 |
426-450 |
Indo-1 (violet[hi-ca]), DAPI |
|
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