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Facscan troubleshooting
- Neither cytometer nor computer will not turn on.
- Make sure that UPS under bench is turned on.
- Computer will not connect to cytometer
- Restart computer
- Cytometer status is NOT READY
- Allow to warm up for 5 minutes.
- Check sheath is full and waste is empty
- If status window says that waste is full and you know it is empty, rap on waste tank to dislodge sensor float.
- Check that tubing leading to tanks is not pinched or obstructed.
- Cytometer status remains at STANDBY
- Fluidics control not turned to "RUN"
- Tube is cracked
- Check that pressure is ON (vent valve UP)
- Check that sheath tank is actually pressurized - it should not be moveable
- Ensure that sheath tank cap is screwed down tightly
- Stuck fluidics - turn to STANDBY and back to RUN
- Status READY, but sample will not run
- No cells in sample
- Clumpy sample - filter it
- Blocked SIP or flow cell - DRAIN/FILL 3 times
- Waste not venting - loosen waste tank cap a little. Report problem
- Sample runs for a few seconds then stops
- Clumpy sample - filter it
- Blocked SIP or flow cell - DRAIN/FILL
- Waste not venting - loosen waste tank cap a little. Report problem.
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