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Facscan troubleshooting
- Neither cytometer nor computer will not turn on.
- Make sure that UPS under bench is turned on.
- Computer will not connect to cytometer.
- Restart computer.
- Cytometer status is "NOT READY".
- Allow to warm up for 5 minutes.
- Check sheath is full and waste is empty.
- If status window says that waste is full and you know it is empty, rap on waste tank to dislodge sensor float.
- Check that tubing leading to tanks is not pinched or obstructed.
- Cytometer status remains at "STANDBY".
- Fluidics control not turned to "RUN"
- Tube is cracked.
- Check that pressure is ON (vent valve UP).
- Check that sheath tank is actually pressurized - it should not be moveable.
- Ensure that sheath tank cap is screwed down tightly.
- Stuck fluidics - turn to STANDBY and back to RUN.
- Status READY, but sample will not run.
- No cells in sample.
- Clumpy sample - filter it.
- Blocked SIP or flow cell - Remove sample, then DRAIN/FILL 3 times.
- Waste not venting - loosen waste tank cap. Report problem.
- Sample runs for a few seconds then stops.
- Clumpy sample - filter it.
- Blocked SIP or flow cell - Remove sample, then DRAIN/FILL 3 times.
- Waste not venting - loosen waste tank cap. Report problem.
- Sample still will not run.
- Air bubbles in sheath filter - purge filter.
- SIP blocked - Set fluidics to "HIGH". Run 100% bleach for 30 sec. followed by at least 2 changes of DI water for 1 minute.
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